Flavobacterium cloacae sp. nov., isolated from waste water.

A Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium, named wh15T, was isolated from the waste water of a municipal wastewater treatment plant in Wuhu, PR China. The taxonomic status of the strain was characterized using a polyphasic taxonomic approach. Growth occurred at 15-45 °C (optimum, 25-40 °C), at pH 5.0-8.0 (optimum, pH 7.0) and with 0-4 % NaCl (optimum, 1-2 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that wh15T represented a member of genus Flavobacterium and shared the highest similarity with Flavobacterium ummariense DS-12T (93.93 %), Flavobacterium ceti 454-2T (93.71 %) and Flavobacterium marinum SW105T (93.63 %). The major respiratory quinone of wh15T was MK-6 and major fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl). The DNA G+C content of wh15T was 32.3 mol%. Phenotypic, phylogenetic and chemotaxonomic evidence indicated that wh15T represents a novel species of the genus Flavobacterium, the name Flavobacterium cloacae is proposed. The type strain is wh15T (=CCTCC AB 2015420T=JCM 31173T).