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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Measures of RNA metabolism rates: Toward a definition at the level of single bonds.
Transcription 2017 March 16
We give an overview of experimental and computational methods to estimate RNA metabolism rates genome-wide. We then advocate a local definition of RNA metabolism rate at the level of individual phosphodiester bonds. Rates of formation and disappearance of individual bonds are unambiguously defined, in contrast to rates of complete transcripts. We show that over previous approaches, the recently developed transient transcriptome sequencing (TT-seq) protocol allows for estimation of metabolism rates of individual bonds with least positional bias.
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