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Application of a dual target PCR-high resolution melting (HRM) method for rapid nontuberculous mycobacteria identification.

Jonathan Hk Chen, Vincent Cc Cheng, Kevin Kk She, Wing-Cheong Yam, Kwok-Yung Yuen
Journal of Microbiological Methods 2017 January
Species differentiation of nontuberculous mycobacteria (NTM) has long been a difficult task in clinical laboratories. This study demonstrated and evaluated a simple and cost-effective method using the real-time PCR with high-resolution melting (PCR-HRM) analysis technique, which could differentiate at least 14 different medically related NTM.

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