Hyperspectral imaging analysis of a photonic crystal bead array for multiplex bioassays.

For multiplex bioassays, one effective strategy is to employ microfluidic chips based on an array of photonic crystal beads (PCBs) that are encoded by their characteristic reflection spectrum (CRS). In this paper, we report a hyperspectral imaging system and algorithms for the high throughput decoding of a PCB array and subsequent detection. The results showed that the decoding accuracy of up to ∼500 PCBs is 98.56% with an excellent ability to extract low-intensity fluorescence intensities. The results also demonstrated hyperspectral imaging techniques which can simultaneously obtain both spatial and spectral information as powerful tools in the analysis of multiplex bioassays or microfluidic chips.