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Identification of a discordant genotype at the D2S1338 locus between Identifiler Plus(®)16 kit and Power Plex(®)21 system kit.
Journal of Forensic and Legal Medicine 2016 November
Genotyping errors can perturb estimates of genetic diversity and adversely affects the reliability of forensic identification, which has become increasingly recognized. During our daily laboratory work, we hit upon a discordant short tandem repeat (STR) genotype at the D2S1338 locus between Identifiler Plus(®)16 system kit and Power Plex(®)21 system kit. To figure out the reason of the discordance, nested PCR was performed to amplify the D2S1338 locus and then the final amplicons were sequenced. We found a G to G/A transition at the position on which caused an allele dropout of Identifiler Plus(®)16 kit.
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