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Prevalence, and virulence determination of Listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction.
Revista da Sociedade Brasileira de Medicina Tropical 2016 September
INTRODUCTION:: This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR).
METHODS:: A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes.
RESULTS:: L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, and inlJ were detected in 100%, 76.26%, and 71% isolates, respectively.
CONCLUSIONS:: This study validated MPCR in the analysis and rapid detection of L. monocytogenes. The role of the genes in pathogenesis of the strains can also be affirmed.
METHODS:: A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes.
RESULTS:: L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, and inlJ were detected in 100%, 76.26%, and 71% isolates, respectively.
CONCLUSIONS:: This study validated MPCR in the analysis and rapid detection of L. monocytogenes. The role of the genes in pathogenesis of the strains can also be affirmed.
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