We have located links that may give you full text access.
JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Conformational Transition of Polyelectrolyte As Influenced by Electrostatic Complexation with Protein.
Biomacromolecules 2016 December 13
Conformation and conformational transitions are essential for the biological and technological functions of natural polyelectrolytes, for example, DNA. This study aims to clarify how the conformational transition of natural polyelectrolyte is affected and tuned by electrostatic complexation with protein as encountered in many biological processes. A model protein/polyelectrolyte system, β-lactoglobulin (β-lg) and κ-carrageenan (κ-car), was used for the investigation. The effect was found to be determined by the molecular state of β-lg/κ-car electrostatic complexation and the molecular weight of protein. β-lg/κ-car complexation in soluble state had a subtle effect on the coil-to-helix transition of κ-car, while that in insoluble state greatly suppressed it. On the basis of the McGhee-Hippel theory, a quantitative model was successfully developed to describe the effect of protein/polyelectrolyte electrostatic complexation on the conformational transition of polyelectrolyte. The model can also provide additional information on the change of tertiary structure of β-lg upon electrostatic complexation with κ-car. Moreover, it was found that β-lg or its hydrolysates with a molecular weight larger than 2000 Da hindered the conformational transition of κ-car, while those with a molecular weight lower than 1000 Da promoted it. The observations offer a promising approach to control the conformational transition and related properties of polyelectrolytes for technological applications.
Full text links
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app