[Application of Multiplex PCR for the Screening Di(b-),Co(a-) Rare Blood Group in Chinese Xinjiang Minorities].

OBJECTIVE: To investigate the distribution of Colton and Diego rare blood group antigens of blood donors in Chinese Xinjiang minorities.

METHODS: A multiplex PCR was applied to screen for alleles antigens Dia and Cob in 1020 randomly selected healthy donors of Chinese Xinjiang minorities by using each 5 samples mixed detection method. The samples in the positive pools were further tested individually. Furthermore, the positive samples, including Di(a+b-) /Di(a+b+) and Co(a-b+) / Co(a+b+) genotypes were tested via 2 PCR-SSP assays for high frequency allele Dib and Coa to get the rare genotypes Di(b-) , Co(a-) .

RESULTS: Among 1020 samples 12 cases with Cob+ allele, 45 cases with Di(a+b+) and 1 case with Di(a+b-) were identified.

CONCLUSION: The frequencies of Dia and Cob alleles are 2.30% and 0.59%, respectively. The information of rare blood donors obtained from the screening can provide a reference for matched blood transfusion, and further enrich the National Rare Blood Bank of China.