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Bactericidal effect of γ-radiation with (137)Cesium in platelet concentrates.
Transfusion and Apheresis Science 2016 December
INTRODUCTION: γ-radiation is a method that was originally designed for inactivation of T lymphocytes in blood and blood components in order to prevent transfusion associated-graft versus host disease (TA-GVHD). Klebsiella pneumoniae, Escherichia coli, Enterococcus faecium and Staphylococcus epidermidis strains are important pathogens in blood banks since they have been related to post-transfusional sepsis. This study was conducted to demonstrate that γ-radiation is effective in reducing the viability of bacteria in platelet concentrates (PC).
MATERIAL AND METHODS: Klebsiella pneumoniae, E. coli, E. faecium and S. epidermidis strains were adjusted at 10(1) to 10(6) CFU/ml and used in artificial contamination assays in PC. Contaminated platelet concentrates were subjected to γ-radiation with doses of 2500 cGy in a (137)Cesium irradiator. The average of surviving bacteria at different bacterial concentrations, logarithmic reduction values (LRV) and bacterial death after γ-radiation percentage was calculated.
RESULTS: Escherichia coli and K. pneumoniae were eliminated in 10(1) to 10(3) CFU; in contrast with 10(4) to 10(6) CFU, the LRV were 2.4, 2.6 and 2.6 for E. coli and 3.3, 2.7 and 3.0 for K. pneumoniae strains at 10(4), 10(5) and 10(6) CFU respectively. For Gram-positive strains, 10(1) CFU in PC, the inactivation post γ-radiation was not completed. Logarithmic reduction values post γ-radiation were 0.8 to 1.2 for E. faecium and S. epidermidis strains respectively.
CONCLUSION: γ-radiation cannot be an alternative for the inactivation of pathogens in PC, because of the bacterial concentration and pathogen nature - being resistant to γ-radiation, the Gram-positive bacteria.
MATERIAL AND METHODS: Klebsiella pneumoniae, E. coli, E. faecium and S. epidermidis strains were adjusted at 10(1) to 10(6) CFU/ml and used in artificial contamination assays in PC. Contaminated platelet concentrates were subjected to γ-radiation with doses of 2500 cGy in a (137)Cesium irradiator. The average of surviving bacteria at different bacterial concentrations, logarithmic reduction values (LRV) and bacterial death after γ-radiation percentage was calculated.
RESULTS: Escherichia coli and K. pneumoniae were eliminated in 10(1) to 10(3) CFU; in contrast with 10(4) to 10(6) CFU, the LRV were 2.4, 2.6 and 2.6 for E. coli and 3.3, 2.7 and 3.0 for K. pneumoniae strains at 10(4), 10(5) and 10(6) CFU respectively. For Gram-positive strains, 10(1) CFU in PC, the inactivation post γ-radiation was not completed. Logarithmic reduction values post γ-radiation were 0.8 to 1.2 for E. faecium and S. epidermidis strains respectively.
CONCLUSION: γ-radiation cannot be an alternative for the inactivation of pathogens in PC, because of the bacterial concentration and pathogen nature - being resistant to γ-radiation, the Gram-positive bacteria.
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