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Colorimetric detection of glucose based on gold nanoparticles coupled with silver nanoparticles.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy 2017 Februrary 16
We have coupled gold nanoparticles (AuNPs) with silver nanoparticles (AgNPs) to assemble a plasmonic sensing platform for colorimetric detection of glucose. In this system, small AuNPs (~4nm) can act as glucose oxidase (GOD) mimic enzyme to catalytically oxidize glucose in the presence of oxygen, producing hydrogen peroxide, which dissolves AgNPs to lead the color changes. Glucose can be detected not only by naked eyes (from yellow to red) but also by spectrophotometer in the concentration range of 5-70μM, with detection limit of 3μM. More importantly, we found that l-cysteine added in the system can markedly improve the selectivity for the detection of glucose. The proposed method was used to application for the detection of glucose in human serum with satisfactory results. This system is simple and low cost without using any enzymes and organic chromogenic agents.
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