Add like
Add dislike
Add to saved papers

Depletion of SHANK2 inhibited the osteo/dentinogenic differentiation potentials of stem cells from apical papilla.

The aim of this study was to investigate the biological function of SHANK2 on the osteo/dentinogenic differentiation potentials of human stem cells from apical papilla (SCAPs). Real-time RT-PCR was used to detect the expression of SHANK2 in human mesenchymal stem cells (MSCs). Small hairpin RNA (shRNA) was used to knockdown the SHANK2 in SCAPs. The knockdown efficiency was determined by real-time RT-PCR and Western Blot. The in vitro osteo/dentinogenic differentiation potentials of SCAPs were investigated using ALP staining, ALP activity, alizarin red staining, quantitative calcium, the expression levels of DSPP, DMP1, RUNX2 and OSX. In vivo transplantation experiments in immunocompromised mice were used to evaluate the capacity of SCAPs to form bone/dentine-like structure. SHANK2 was highly expressed in dental tissue-derived MSCs compared with cells of other origins. Silencing of SHANK2 inhibited the ALP activity, mineralization, and the expressions of DSPP, DMP1, RUNX2 and OSX in SCAPs. Furthermore, in vivo transplantation experiments indicated that knock-down of SHANK2 in SCAPs generated less bone/dentin-like mineralized tissue compared with the control group. The present study demonstrated that depletion of SHANK2 inhibited the osteo/dentinogenic differentiation potentials in SCAPs, explored the new function of SHANK2, and provided useful information to elucidate the molecular mechanism underlying directed differentiation in dental tissue-derived MSCs.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app