Lysobacter rhizophilus sp. nov., isolated from rhizosphere soil of mugunghwa, the national flower of South Korea.

A Gram-staining-negative, aerobic, motile, rod-shaped, catalase- and oxidase-negative strain with one polar flagellum, designated THG-YS3.6T, was isolated from rhizosphere soil of a mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-37 °C (optimum 25-30 °C), at pH 6-8 (optimum 7.0) and with 0-2.0 % NaCl (optimum 1 %). The isoprenoid quinone was ubiquinone-8 (Q-8). The major cellular fatty acids were iso-C11  : 0, iso-C11 : 0 3-OH, iso-C15 : 0, iso-C16 : 0, C16 : 1ω7c alcohol, C16 : 0, iso-C17 : 0 and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unknown phospholipid, one unknown lipid and three unknown aminolipids. The DNA G+C content of strain THG-YS3.6T was 65.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.6T were identified as Lysobacter yangpyeongensis KACC 11407T (98.7 %), Lysobacter oryzae KCTC 22249T (98.0 %), Lysobacter niabensis KACC 11587T (97.6 %) and Lysobacter terrae KACC 17646T (97.1 %). The DNA-DNA relatedness values between strain THG-YS3.6T and L. yangpyeongensis KACC 11407T, L. oryzae KCTC 22249T, L. niabensis KACC 11587T and L. terrae KACC 17646T were 53.8±1.0 %, 12.9±1.2 %, 10.9±0.6 % and 7.0±1.9 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.6T represents a novel species of the genus Lysobacter, for which the name Lysobacter rhizophilus sp. nov. is proposed. The type strain is THG-YS3.6T (=KCTC 52082T=CCTCC AB 2015358T).