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Use of antioxidants in the analysis of vatamins A and E in mammalian plasma by high performance liquid chromatography.
Lipids 1983 November
A simple, sensitive, quantitative method for the simultaneous assay of retinol, α-tocopherol and γ-tocopherol in rat, guinea pig, monkey and human plasma was developed by using high performance liquid chromatography. It was found that antioxidant was required to stabilize the fat-soluble vitamins in the plasma of rats. The effect of several antioxidants on the recovery of fat-soluble vitamins was evaluated. Results showed that 0.125% butylated hydroxytoluene (BHT) in ethanol and 0.025% BHT in heptane yielded recoveries >95% in 0.1 ml plasma.
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