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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Analysis and quality control of carbohydrates in therapeutic proteins with fluorescence HPLC.
Biochemical and Biophysical Research Communications 2016 September 17
Conbercept is an Fc fusion protein with very complicated carbohydrate profiles which must be carefully monitored through manufacturing process. Here, we introduce an optimized fluorescence derivatization high-performance liquid chromatographic method for glycan mapping in conbercept. Compared with conventional glycan analysis method, this method has much better resolution and higher reproducibility making it excellent for product quality control.
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