[Effect and mechanism of interleukin-8 expression induced by wood smoke particles in primary human airway epithelial cells].

OBJECTIVE: To investigate the effect and mechanism of interleukin-8 expression induced by wood smoke particles (WSP) in primary human airway epithelial cells.

METHODS: Primary human bronchial epithelial cells (HBEC) were collected through fiberbronchoscopic brushing and incubated with different concentration of WSP (0, 12.5, 25.0, 50.0, 100.0 μg/ml). Levels of IL-8 protein in cell culture supernatant were measured using enzyme-linked immune sorbent assay. The specific inhibitors for epidermal growth factor receptor (EGFR) and p38 kinase signaling pathways were employed to pretreat HBEC, respectively, prior to incubation with 100 μg/ml WSP to investigate the mechanism of IL-8 expression induced by WSP.

RESULTS: The expression of IL-8 was significant increased in a dose-dependent manner after exposure HBEC to different concentration of WSP for 24h. The levels of IL-8 expression were (4546.67±1421.42) ρg/ml in (PD153035+WSP) group and (2803.33±865.00) ρg/ml in (SB203580+WSP) group respectively, which were significant decreased compared with the level of (12896.67±1373.11) ρg/ml in WSP group (P<0.05).

CONCLUSION: WSP could induce IL-8 expression by means of EGFR and p38 signaling pathways.