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Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Self-Aggregating Deep Cavitand Acts as a Fluorescence Displacement Sensor for Lysine Methylation.
Journal of the American Chemical Society 2016 August 32
A dual-mode aggregative host:guest indicator displacement sensing system has been created for the detection of trimethylated peptides and determination of histone demethylase activity. The combination of selective recognition of suitably sized trimethylammonium salts and reversible lipophilic aggregation of the host:guest complex provides a unique quenching mechanism that is not only dependent on affinity for sensitivity but the lipophilicity of the indicator. In addition, aggregation can be controlled by the application of chaotropic anions in the mixture, allowing a second level of discrimination between hard lysine groups and softer trimethyllysines.
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