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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Purification of infectious and non-infectious chlamydial particles using iodixanol for density gradient preparation.
Journal of Microbiological Methods 2016 September
Chlamydiae are obligate intracellular bacteria with two distinct morphological stages, the infectious elementary bodies (EBs) and non-infectious reticulate bodies (RBs). Here we describe a rapid and straightforward protocol for the purification of EBs and RBs involving special density gradients. It has been successfully applied to three chlamydial species.
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