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The Project Of Another Low-Cost Metaphase Finder.
Radiation Protection Dosimetry 2016 December
The most popular and 'gold standard' phenomenon in Biological dosimetry is the appearance of dicentric chromosomes in metaphase in white blood cells. The metaphase finder is a tool for biological dosimetry that finds metaphase cells on slide glasses. The author and a software company were using new special software that was faster than conventional systems. A Nikon Eclipse Ni-E microscope with motorised X-Y stage, 4× objective lens and 1920 × 1024 pixels colour camera for hardware were used. The software uses mathematical morphology filters. The new system was compact and low-priced. And the remarkable point is, this system can be applicable not only to human blood, but also to non-human samples. The speed was 208-236 s per 5 × 20 mm area, while capturing 378 images, which achieved the aim of the project. The false-positive ratio achieved below 5% in some slides.
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