[Observation on growth status of Blastocystis hominis in three media].

OBJECTIVE: To observe the growth situation of Blastocystis hominis in vitro and select the optimal method for cultivation of B. hominis in different media.

METHODS: Ten positive stools with B. hominis were inoculated in three different media for cultivating, namely 1640, Jone's medium and vitro medium. And the stools with good growth status and high quantities of B. hominis were chosen to inoculate in the three media with equal amount after subcultivation, and the number of B. hominis was counted every 24 h for ten days, and the morphological changes and growth status were also observed.

RESULTS: The densities of B. hominis in the 1640 and Jone's medium were higher than that in the vitro medium 48 h after the inoculation. The same stool sample was inoculated to the three different media and observed for ten days, and the results indicated that the growth of B. hominis presented regular changes in the three media, the growth peaks were on the third, sixth and ninth day post inoculation; and the density of B. hominis was the highest in the Jone's medium. The morphology of B. hominis was the clearest and most dynamic in the vitro medium, while various reproductive forms were observed in the Jone's medium.

CONCLUSION: Jone's medium is suitable for the growth of B. hominis and can be the first choice for the cultivation of B. hominis in vitro, and vitro medium is the best medium for observing the growth situation of B. hominis.