Ameliorating effect of Kalpaamruthaa on altered energy metabolism in BCR-ABL(+) cell line induced leukemic mouse model.

OBJECTIVE: To evaluate the protective effect of Kalpaamruthaa (KA), a modified Siddha preparation, in BCR-ABL(+) leukemic mouse model.

METHODS: BCR-ABL leukemia was induced in 6-10-week-old female BALB/c mice by a single tail vein injection of the 12B1 cell line. Leukemia-induced animals were treated with KA at a dosage of 200 mg/kg body weight dissolved in 0.5 mL of olive oil for 14 days by gastrogavage. Imatinib mesylate was used as the control drug. Glycolytic, gluconeogenic, mitochondrial, tricarboxylic acid cycle and respiratory chain enzymes in the spleen and liver of mouse were compared between the control and experiment groups by biochemical assays.

RESULTS: Leukemia-bearing mice showed a significant increase in glycolytic enzymes and a signififi cant decrease in gluconeogenic enzymes, tricarboxylic acid cycle and respiratory chain enzymes as compared with control animals. Treatment with KA signififi cantly reversed the changes seen in the levels of the glycolytic enzymes, gluconeogenic enzymes and mitochondrial enzymes.

CONCLUSION: The presence of various flfl avonoids and polyphenols in the drug KA might have resulted in the amelioration of altered glucose metabolism resulting in the regression of leukemia.