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Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis.

Xiaohong Zeng, Hui Li, Rongrong Zheng, Natalia Kurepina, Barry N Kreiswirth, Xilin Zhao, Ye Xu, Qingge Li
Journal of Clinical Microbiology 2016 September
We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing.

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