Solid-liquid Extraction of Phenolics from Red Grape Skins.

For the characterization of grape cultivars, the profile and content of flavonoids are important because these compounds have an impact on grape and wine quality. A new extraction method for the recovery of flavonoids, e.g. anthocyanins, flavonols and flavan-3-ols from grape skins was developed. The optimization of solid-liquid extraction of flavonoids was conducted, with respect to the type of the organic solvent and its percentage in the extraction solvent as well as the extraction temperature and extraction time, using response surface methodology. Optimal conditions were obtained by using extraction solvent composed from acetonitrile:water:formic acid (20:79:1; v/v/v), at an extraction temperature of 50 °C, an extraction time of 1 h in a single-step extraction and with a solid-to-solvent ratio of 1:80 g mL(-1) (125 mg of grape skin powder and 10 mL of extraction solvent). The new optimal extraction method is inexpensive, simple, fast, accurate and selective for the recovery of simple flavonoids.