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Letter
Research Support, Non-U.S. Gov't
The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile.
ACS Synthetic Biology 2016 December 17
Clostridium difficile is an opportunistic pathogen and the main cause of antibiotic-associated diarrhea. Adherence of C. difficile to host cells is modulated by proteins present on the bacterial cell surface or secreted into the environment. Cleavage of collagen-binding proteins is mediated by the zinc metalloprotease PPEP-1, which was identified as one of the most abundant secreted proteins of C. difficile. Here, we exploit the PPEP-1 signal sequence to produce novel secreted enzymes. We have constructed two functional secreted reporters, AmyEopt and sLucopt for gene expression analysis in C. difficile. AmyEopt extracellular activity results in starch degradation and can be exploited to demonstrate promoter activity in liquid or plate-based assays. sLucopt activity could reliably be detected in culture supernatant when produced from an inducible or native promoter. The secreted reporters can be easily assessed under aerobic conditions, without the need of complex sample processing.
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