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Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Catch and Release DNA Decoys: Capture and Photochemical Dissociation of NF-κB Transcription Factors.
ACS Chemical Biology 2016 June 18
Catch and release DNA decoys (CRDDs) are a new class of non-natural DNA probes that capture and dissociate from DNA-binding proteins using a light trigger. Photolytic cleavage of non-natural nucleobases in the CRDD yields abasic sites and truncation products that lower the affinity of the CRDD for its protein target. Herein, we demonstrate the ability of the first-generation CRDD to bind and release NF-κB proteins. This platform technology should be applicable to other DNA-binding proteins by modification of the target sequence.
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