We have located links that may give you full text access.
Journal Article
Research Support, Non-U.S. Gov't
Relationships between leptin, KiSS-1/GPR54 expression and TSH secretion from pituitary cells of pubertal ewes in vitro.
Research in Veterinary Science 2016 April
Kisspeptin and leptin play a crucial role in the puberty of sheep as they initiate the activity of hypothalamic-pituitary-ovarian axis. Also hormones of thyrotropic axis are probably involved in this process. The aim of study was to analyze the impact of leptin on kisspeptin-10 secretion as well as kisspeptin-1 and G protein-coupled receptor (GPR54) mRNA expression in pituitary cells of pubertal ewes in vitro. The influence of kisspeptin on TSH secretion was also examined. Cells were cultured in McCoy's 5A medium without hormones; with 10(-10)-10(-5)M of leptin; with 10(-11)-10(-5)M of kisspeptin-10; with peptide 234 (10(-7)M, antagonist of GPR54) or 10(-11)-10(-5)M of kisspeptin-10 and peptide 234. Then, kisspeptin-10 and TSH secretion as well as KiSS-1 and GPR54 expression were analyzed. We found that leptin directly affected kisspeptin-10 secretion and kisspeptin-1/GPR54 expression in pituitary cells of pubertal ewes. Kisspeptin-10 did not change TSH secretion, except exerting a short-term influence after 2h.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app