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[Tubacin promotes Foxp3 expression and suppressive function of mouse CD4+;CD25+; regulatory T cells].
Xi Bao Yu Fen Zi Mian Yi Xue za Zhi = Chinese Journal of Cellular and Molecular Immunology 2016 March
OBJECTIVE: To observe the changes of fork head transcription factor (Foxp3) expressions and suppressive functions of mouse regulatory T cells (Tregs) after stimulated by tubacin (histone deacetylase 6 inhibitor) in vitro.
METHODS: CD4(+) CD25(+) T and CD4(+) CD25(-) T cells were gained from C57BL/6J mouse spleen lymphocytes by double positive magnetic bead sorting method. Natural Tregs (nTregs) and transforming growth factor β1 (TGF-β1)-induced Tregs (iTregs) were stimulated by tubacin in vitro. Foxp3 expression of each group was identified by reverse transcription PCR, and immunosuppressive activities of each group were tested by the mixed lymphocyte culture (MLC).
RESULTS: Foxp3 expression of nTregs and iTregs were significantly enhanced after stimulated with tubacin. The tubacin induced CD4(+) CD25(+) Foxp3(high) Tregs significantly inhibited syngeneic CD4(+) CD25(-) T cell activation.
CONCLUSION: Tubacin can upregulate Foxp3 expression of CD4(+) CD25(+) Tregs and enhances their cellular immunosuppressive capability.
METHODS: CD4(+) CD25(+) T and CD4(+) CD25(-) T cells were gained from C57BL/6J mouse spleen lymphocytes by double positive magnetic bead sorting method. Natural Tregs (nTregs) and transforming growth factor β1 (TGF-β1)-induced Tregs (iTregs) were stimulated by tubacin in vitro. Foxp3 expression of each group was identified by reverse transcription PCR, and immunosuppressive activities of each group were tested by the mixed lymphocyte culture (MLC).
RESULTS: Foxp3 expression of nTregs and iTregs were significantly enhanced after stimulated with tubacin. The tubacin induced CD4(+) CD25(+) Foxp3(high) Tregs significantly inhibited syngeneic CD4(+) CD25(-) T cell activation.
CONCLUSION: Tubacin can upregulate Foxp3 expression of CD4(+) CD25(+) Tregs and enhances their cellular immunosuppressive capability.
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