Streptomyces arcticus sp. nov., isolated from frozen soil.

Actinomycete strain ZLN234(T) was isolated from a frozen soil sample, which was collected from a glacier front in the Arctic. Chemotaxonomic and morphological characteristics were found to be typical of members of the genus Streptomyces. The strain formed a distinct phyletic line in the 16S rRNA gene tree and was closely related to Streptomyces olivochromogenes ATCC 3336(T) (98.63 % similarity) and Streptomyces aureus B7319(T) (98.56 %). Phylogenetic analysis based on the gyrB gene also showed clearly that strain ZLN234(T) was different from all recognized Streptomyces species. The whole-cell sugars were galactose, glucose, ribose and rhamnose. The whole-cell-wall amino acids included alanine, glutamine, glycine and meso-diaminopimelic acid with a minor amount of ll-diaminopimelic acid. The characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and unknown phospholipids. The major menaquinones were MK-9(H10), MK-9(H8) and MK-9(H4). The major cellular fatty acids comprised anteiso-C15 : 0, iso-C16 : 0 and C16 : 0 with a small amount of iso-C16 : 1ω7 and anteiso-C14 : 0. The DNA G+C content of strain ZLN234(T) was 77.5 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, strain ZLN234(T) ( = CCTCC AA 2015005(T) = DSM 100713(T)) is the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces arcticus sp. nov. is proposed.