[Determination of Amino Acids in Pearl Powder and Conch Powder from Different Origins by HPLC with Pre-column Derivation].

OBJECTIVE: To establish an analysis method for determination of amino acids in pearl powder and conch powder from different origins by HPLC with pre-column derivation.

METHODS: Hydrolytic amino acids were extracted by acid hydrolysis method,then derivatized with phenyl isothiocyanate (PITC). And the samples were analyzed on a C, column with gradient elution of 0.1 mol/L sodium acetate solution (adjusted to pH 6. 5) (A) and acetonitrile (B) at the flow rate of 1. 0 mL/min. The detected wavelength was 254 nm.

RESULTS: Amino acids derivative solution remained stable in 24 hours. The response was linear for seven amino acids with a correlation coefficient r >0. 9995. The average recoveries were 93. 16% - 103. 31%.

CONCLUSION: The method is sensitive with good accuracy and repeatability, which is useful for the determination of amino acids in pearl powder and conch powder from differei origins.