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Journal Article
Research Support, N.I.H., Extramural
Whole Mouse Brain Image Reconstruction from Serial Coronal Sections Using FIJI (ImageJ).
Current Protocols in Neuroscience 2015 October 2
Whole-brain reconstruction of the mouse enables comprehensive analysis of the distribution of neurochemical markers, the distribution of anterogradely labeled axonal projections or retrogradely labeled neurons projecting to a specific brain site, or the distribution of neurons displaying activity-related markers in behavioral paradigms. This unit describes a method to produce whole-brain reconstruction image sets from coronal brain sections with up to four fluorescent markers using the freely available image-processing program FIJI (ImageJ).
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