JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Add like
Add dislike
Add to saved papers

Synthesis of Site-Specifically Phosphate-Caged siRNAs.

Photolabile small interfering RNA (siRNA) oligonucleotide duplexes are becoming a powerful tool for photoregulation of gene expression through an RNA interference (RNAi) mechanism. Terminal or statistical labeling of siRNAs has been previously achieved. Recently, we have shown a new strategy for site-specific incorporation of a photolabile group (1-(2-nitrophenyl)ethyl [NPE]) at any phosphate position of siRNA strands for photomodulation of their gene-silencing activity. In this unit, we first describe in detail the syntheses of four new NPE protected nucleoside phosphoramidites (dA 0, dG 0, dC 0, dT 0) and 2-cyanoethyl-1-(2-nitrophenyl)ethyl-N,N'-diisopropylphosphoramidite (N 0). They are then site specifically incorporated into any position of RNA oligonucleotides according to standard phosphoramidite chemistry. Phosphate-caged siRNA duplexes are then prepared by hybridization of single-stranded RNAs containing the 1-(2-nitrophenyl)ethyl caging moiety on the phosphate group with their complementary RNA.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app