Neither insufficiency nor overexpression of sac1 affects the accumulation of Aβ42 in Drosophila expressing Ab42.

OBJECTIVE: We investigated the effects of genetic down- and up-regulation of sac1 expression on Aβ42 accumulation and the associated neural deficits in flies with direct expression of arctic mutant Aβ42 (Aβarc) in the neurons of GF pathway.

MATERIALS AND METHODS: We genetically down-regulated and up-regulated the level of sac1, encoding a major phosphoinositide phosphatases in a disease model, in which arctic mutant Aβ42 is directly expressed in the neurons of a neural pathway of adult fruit flies.

RESULTS: We conducted a time-course analysis of Aβ42 level in the model and found an age-dependent elevation of Aβ42 accumulation, closely correlated to the age-dependent decline of climbing ability in the model flies. Neither sac1 insufficiency nor sac1 over-expression significantly changed the three phenotypes.

CONCLUSIONS: We found that the alterations of sac1 expression did not change Aβ42 accumulation and neural deficits in the model.