Does the liposuction method influence the phenotypic characteristic of human Adipose-Derived Stem Cells?

ASCs possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of this study was to analysis of 242 surface markers and determine the difference in the phenotypic pattern between ASCs obtained during mechanically and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanically and ultrasound-assisted liposuction and cultured in standard medium to the second passage. Differentiation potential and markers expression was performed to confirm the mesenchymal nature of cells. Then, the BD LyoplateTM Human Cell Surface Marker Screening Panel was used. Results shown that both population of ASCs are characterized by high expression of markers specific for adipose derived stem cells: CD9, CD10, CD34, CD44, CD49d, CD54, CD55, CD59, CD71 and low expression of CD11a, CD11c and CD144. Moreover, we have noticed significant differences in antigen expression in 58 markers of the 242 studied. Presented study shows for the first time that different liposuction method is not a significant factor which can influence the expression of human adipose-derived stem cells surface markers.