Biological differences between native and cultured mesenchymal stem cells: implications for therapies.

We describe the current knowledge of the surface marker phenotype of native bone marrow mesenchymal stem/stromal cells (MSCs) in humans and in mouse models, highlighting similarities in the MSC marker "signature" between the two species. The chapter proceeds to discuss the published literature pertaining to native MSC topography and their interactions with hematopoietic stem cells and their progeny, as well as with blood vessels and nerve endings. Additionally, the chapter describes phenotypic and functional "drifts" that occur in MSC preparations as they are taken out of their native bone marrow microenvironment and induced to proliferate in vitro (in the presence of animal or human serum). We propose that the understanding of the biology of MSCs in their native niches in the bone marrow could lead to future developments in the treatment of hematological diseases such as multiple myeloma. Additionally, this knowledge would assist in the development of more "natural" MSC culture conditions, best preserving MSC functionality including their homing potential in order to optimize MSC transplantation in the context of graft-versus-host and other diseases.