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English Abstract
Journal Article
[Histochemical study using lectin and anti-human von Willebrand factor antibody of oral hemangioma].
Twenty nine cases of oral hemangiomas composed of capillary hemangioma, cavernous hemangioma and pyogenic granuloma were examined with lectin histochemistry using ulex europaeus agglutinin I, ricinus communis agglutinin I, wheat germ agglutinin, concanavalin A, dolichos biflorus agglutinin, soybean agglutinin and peanut agglutinin, and immunohistochemistry using anti von Willebrand factor antibody in order to understand the nature of each endothelial cell. The following results were obtained; 1. UEA-I that specifically bound alpha-L-fucose was observed mainly in the endothelial cells of capillary hemangioma and hemangioma of granulation tissue type. 2. RCA-I which had specific affinity to beta-D-galactose was so identified in all endothelial cells of all type hemangiomas and normal blood vessels that it was supposed to be a useful marker of the cells. 3. WGA with specific affinity to N-acetyl-glucosamine was also found in various endothelial cells. 4. Con A which had specific affinity to alpha-D-mannose was not observed in various endothelial cells. 5. DBA, SBA and PNA that were specific bound up with N-acetyl-galactosamine were seen in the normal micro-vessels, especially in subepithelial regions. 6. Immunohistochemically, von Willbrand factor was identified chiefly in the endothelial cells of normal blood vessels and cavernous hemangioma, but it was not found in the cells of capillary hemangioma and pyogenic granuloma which had no differentiation in the functional point of view.
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