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JOURNAL ARTICLE
RESEARCH SUPPORT, N.I.H., INTRAMURAL
Measurement of the temperature of the resting rotor in analytical ultracentrifugation.
Analytical Biochemistry 2014 August 2
Accurate measurements of rotor temperature are critical for the interpretation of hydrodynamic parameters in analytical ultracentrifugation. We have recently developed methods for a more accurate determination of the temperature of a spinning rotor using iButton temperature loggers. Here we report that the temperature measured with the iButton on the counterbalance of a resting rotor, following thermal equilibration under high vacuum, closely corresponded to the temperature of the spinning rotor with a precision better than 0.2°C. This strategy offers an inexpensive and straightforward approach to monitor the accuracy of the temperature calibration and determine corrective temperature offsets.
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