[Identification of proteins interacted with Bat3 using tandem affinity purification].

OBJECTIVE: To identify the specific protein interactions involved in Bat3-mediated apoptosis.

METHODS: Tandem affinity purification (TAP) was utilized to investigate Bat3-protein interactions, during which full-length human Bat3 fused with Strep2 and FLAG tag as a bait was used to screen the specific protein-protein interactions. The isolated proteins were identified with mass spectrometry.

RESULTS: TAP studies showed that Ubl4A was identified as a Bat3-binding partner. Further investigation using co-immunoprecipitation confirmed that Bat3 was associated with Ubl4A.

CONCLUSION: TAP was successfully established and is suitable for isolating the binding partners of Bat3.