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Research Support, Non-U.S. Gov't
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Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy.

D D-U Li, S Poland, S Coelho, D Tyndall, W Zhang, J Richardson, R K Henderson, S M Ameer-Beg
Conference Proceedings : Annual International Conference of the IEEE Engineering in Medicine and Biology Society 2013
We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13µm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.

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