[Effect on different concentrations of exogenous hormones on baicalin in Scutellaria baicalensis callus].

OBJECTIVE: To determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones.

METHOD: HPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions: ODS column was adopted, with methanol-water-phosphate (47: 53: 0.2) as the mobile phase. The flow velocity was 1 mL x min(-1), the detective wavelength was 280 nm, and the temperature of column was room temperature.

RESULT: S. baicalensis callus induced by 6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) showed the highest baicalin content, up to 49.78 mg x g(-1).

CONCLUSION: The experiment is such a simple, rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.