ENGLISH ABSTRACT
JOURNAL ARTICLE
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[The optimization of conditions of diagnostic fluorescent monoclonal immunoglobulins production to identify comma bacillus of serogroups O1 and O139].

The article considers, the issue of producing the species-specific fluorescent monoclonal immunoglobulins to detect comma bacillus of serogrups O1 and O139 in the reaction of direct immunfluorescence. It is established that not only ascitic but culture fluid too can be the source of monoclonal antibodies for producing fluorescent conjugates. The optimal conditions are selected to produce the fluorescent monoclonal immunoglobulins-monoclonal antibodies. The corresponding producing techology can be reproduced at any time in view of availability of hybrid-producers of monoclonal antibodies O1 and monoclonal antibodies O139 in the institute cryodepositoty. The results of testing the fluorescent preparations on homologous and heterologous strains demonstrated their strict specificiy and high sensibility regarding comma bacillus of serogroups O1 and O139. The new preparations favor significant increase of effectiveness of diagnostics of V. cholerae O1 and O139.

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