Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with American cutaneous leishmaniasis.

João Guilherme Lino da Silva, Thiago Miranda da Silva, Eduardo de Figueiredo Peloso, George Luiz Lins Machado-Coelho, Wilson Mayrink, Marília Caixeta Franco Ariosa, Paulo Márcio de Faria e Silva, Marcos José Marques

Revista da Sociedade Brasileira de Medicina Tropical 2012 March

INTRODUCTION: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples.

METHODS: Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR).

RESULTS: Results were evidenced by bands of approximately 120, 720, and 670 bp for L150/L152, 13Y/13Z, and snPCR, respectively. L150/L152, 13Y/13Z, and snPCR positivity indexes were 76.9, 56.4, and 9.2 (p>0.05), respectively, for suspected and 93.7, 68.7, and 84.4 (p<0.05), respectively, for confirmed.

CONCLUSIONS: Preliminary results showed that these assays, mainly L150/L152 and snPCR, can detect Leishmania DNA and carry potential on laboratory diagnosis of leishmaniasis.

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