Arenimonas daechungensis sp. nov., isolated from the sediment of a eutrophic reservoir.

A gram-negative, non-motile, non-spore-forming and rod-shaped bacterial strain, CH15-1(T), was isolated from a sediment sample taken from Daechung Reservoir, South Korea, during the late-blooming period of cyanobacteria. Strain CH15-1(T) grew optimally at pH 7.0 and 30 °C. A phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain CH15-1(T) belongs to the genus Arenimonas with the similarity range from 92.6-97.4 % and is closely related to Arenimonas oryziterrae YC6267(T) (97.4 %), Arenimonas composti TR7-09(T) (95.4 %), Arenimonas metalli CF5-1(T) (94.7 %), Arenimonas malthae CC-JY-1(T) (94.6 %) and Arenimonas donghaensis HO3-R19(T) (92.6 %). However, the DNA-DNA hybridization between strain CH15-1(T) and the closest strain, Arenimonas oryziterrae YC6267(T), was 8.9-12.9 %. The DNA G+C content was 63.9 mol% compared to A. oryziterrae YC626(T), 65.8 mol%. Strain CH15-1(T) included Q-8 as the major ubiquinone and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmonomethylethanolamine as the major polar lipids. The major fatty acids (>5 %) were iso-C(15 : 0), iso-C(16 : 0), iso-C(14 : 0), iso-C(11 : 0) 3-OH, iso-C(17 : 0) and summed feature 9 (iso-C(17 : 1)ω9c and/or C(16 : 0) 10-methyl). On the basis of phylogenetic, phenotypic and genetic data, strain CH15-1(T) was classified in the genus Arenimonas as a member of a novel species, for which the name Arenimonas daechungensis sp. nov. is proposed. The type strain is CH15-1(T) ( = KCTC 23553(T) = DSM 24763(T)).