[Correlation of adiponectin gene SNP +45T/G polymorphism and premature coronary heart disease of Han ethnic males].

OBJECTIVE: To examine the relationship between single nucleotide polymorphism (SNP) of adiponectin (APN) locus +45T/G and Han male patients with premature coronary artery heart disease (pCAD).

METHODS: A total of 423 male patients of Han ethnic group (< 55 yr old) undergoing coronary arteriography were recruited. Among them, 358 patients were diagnosed as pCAD while another 65 normal control (NC). All subjects were genotyped for adiponectin gene SNP +45 by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.

RESULTS: Statistical differences of TG/GG genotype ratio were observed between two groups. The TG/GG genotype ratio in the pCAD group was 51.1% versus 35.4% in the NC group (χ(2) = 5.45, P = 0.022). After an adjustment of conventional risk factors, such as age, body mass index (BMI) and hypertension, the adiponectin gene SNP +45TG/GG genotype was strongly associated with Han ethnic group male pCAD by binary logistic regression analysis (OR = 1.843, P = 0.035, 95%CI: 1.045 ∼ 3.250). And there were statistical differences between TT genotype and TG/GG genotype among pCAD subjects in the following factors: BMI, total cholesterol (TC), low-density lipoprotein-C (LDL-C) and APN level (P < 0.05). By Pearson correlation analysis, APN was negatively correlated with TC, LDL-C and SNP +45T/G polymorphism.

CONCLUSION: The SNP of adiponectin locus +45T/G is associated with male pCAD. And TG/GG genotype is a possible predisposing gene for Han ethnic group male pCAD.