JOURNAL ARTICLE
RESEARCH SUPPORT, N.I.H., EXTRAMURAL
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Microwave-assisted method for fabrication of carbohydrate cluster microarrays on 3-dimensional hydrazide-dendrimer substrate.

We present here a method for construction of carbohydrate cluster microarrays. This technology utilizes a 3-dimensional poly(amidoamine) starburst dendrimer monolayer assembled on glass surface, which is functionalized with terminal hydrazide groups for site-specific coupling of carbohydrates without prior chemical derivatization. Microwave radiation energy is applied to accelerate carbohydrate coupling on chips. Since this bioarray platform is designed to present sugar chains in defined orientation and cluster configurations, it is particularly suitable for exploration of the structural and conformational diversities of glycoepitopes and their functional properties.

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