[Contribution to the mobile phase selection for HPLC separation in order to make the identification and quantitative determination using the UV-VIS detector].

UNLABELLED: The aim of this study is to elaborate a HPLC with UV detection method for the analysis of some AINS, method which can be transferred in a system with mass spectrometry detection.

MATERIAL AND METHODS: More AINS drugs (diclofenac sodium, ibuprophen, ketoprofen, meloxicame, piroxicame, tenoxicame, nimesulide, phenylbutazone and indomethacin) were studied by UV spectrophotometry and by HPLC.

RESULTS: The UV spectra of the studied AINS were recorded in pure solvents or in a mixture of solvents in order to establish the absorbance that will be used for HPLC detection in UV. To establish the optimum chromatographic parameters, the influence of the flow and of the composition of the mobile phase (both of the aqueous and organic phases) were studied too.

CONCLUSIONS: The proposed procedure is easy and relatively with low cost regarding the MS detection.