[Preliminary study on the establishment of the animal model and biological activity of human being meibomian gland carcinoma].

OBJECTIVE: To explore the methods to establish a xenografted model of meibomian gland carcinoma in nude mice, then find the best method to establish the animal model. The last purpose of this study was to establish a suitable animal model for clinic therapy and basic research of meibomian gland carcinoma.

METHODS: Small pieces of meibomian gland carcinoma tissue from four patients were transplanted subcutaneously in the flanks of eight nude mice through a skin incision. Nature of transplantation tumor including formation, growth and size were observed. The transplanted tumors were stained with hematoxylin-eosin, Sudan III and Immunohistochemistry.

RESULTS: The human meibomian gland carcinoma tissue can survived in nude mice. The pathologic examination revealed that the transplanted tumor was arranged in mass. The tumor cells were round or multisided. The proportion of the nuclei and cytoplasm was imbalance. Nuclear division could be easily observed. The test of Sudan III was positive. Immunohistochemical observation: the results showed that both CK and EMA were positive.

CONCLUSIONS: The subcutaneously xenotransplanted tumor model of human meibomian gland carcinoma with tissue explant in nude mice was successfully established. The transplanted tumor maintained the properties of human meibomian gland carcinoma.