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Immobilized carbonic anhydrase in a membrane lung for enhanced CO2 removal.
ASAIO Transactions 1990 July
The potential use of carbonic anhydrase (CA), immobilized within membrane lungs, to accelerate the removal of CO2 from blood was investigated. Using a variation on the technique of Chang, 500 micrograms/mL of CA was encapsulated in 5-20 microns cellulose nitrate microcapsules which were then immobilized onto a 0.1 m2 silicone rubber membrane. Using an in vitro test circuit, 0.68 mmol/m2/min of CO2 was removed from venous blood through an untreated membrane at steady state. With the CA-immobilized membrane, CO2 removal was enhanced to 1.09 mmol/m2/min. This technique has the potential of greatly improving the CO2 efficiency of commercial membrane lungs for ECCOR.
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