JOURNAL ARTICLE
RESEARCH SUPPORT, N.I.H., EXTRAMURAL
RESEARCH SUPPORT, NON-U.S. GOV'T
Add like
Add dislike
Add to saved papers

Expression of alpha-crystallins in human sebaceous carcinoma of the eyelid.

PURPOSE: Crystallins are molecular chaperones that protect various cells from apoptosis. This study used immunohistochemistry to examine the expression of alphaA-crystallin and alphaB-crystallin in human sebaceous carcinoma tissues.

METHODS: Nine patients with sebaceous carcinoma of the eyelid underwent excision of the eyelid or orbital exenteration. Formalin-fixed, paraffin-embedded tissue sections were submitted for hematoxylin-eosin staining and immunohistochemistry with anti-alphaA- and alphaB-crystallin antibodies.

RESULTS: In the noncancerous eyelid, crystallins were weakly and homogeneously expressed in the Meibomian gland lobules. In eyelids with sebaceous carcinoma, alphaA-crystallin and alphaB-crystallin were highly expressed in 4 cases, where the cytoplasmic immunoreactivity was heterogeneously detected in the tumor cells. Three and 2 cases were shown to express low levels of alphaA-crystallin and alphaB-crystallin, respectively. A statistically significant correlation was observed between expression levels of alphaA-crystallin and alphaB-crystallin in sebaceous carcinomas (p=0.012). All sebaceous carcinoma tissues contained mitotic tumor cells, which showed predominantly perinuclear immunoreactivity of alphaB-crystallin. The mitotic rate of tumor cells was 5.85+/-1.76 and 10.72+/-1.64 in high and moderate/low alphaB-crystallin-expressing cases, respectively. The number of mitotic cells was significantly higher in moderate/low alphaB-crystallin-positive than in high-positive cases (p<0.01).

CONCLUSIONS: Crystallins are expressed in human sebaceous carcinomas including mitotic cells. Expression of alphaB-crystallin correlated with low number of mitotic tumor cells, suggesting that alphaB-crystallin may play a potential role in the regulation of tumor cell proliferation.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app