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Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
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Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

Stephen J Pettitt, Qi Liang, Xin Y Rairdan, Jennifer L Moran, Haydn M Prosser, David R Beier, Kent C Lloyd, Allan Bradley, William C Skarnes
Nature Methods 2009 July
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

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