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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Formation of gutingimycin: analytical investigation of trioxacarcin A-mediated alkylation of dsDNA.
Analytical and Bioanalytical Chemistry 2008 Februrary
Formation and fragmentation of recognition complexes between trioxacarcin A and various DNA sequences were examined by temperature-dependent UV and CD spectroscopy, HPLC analysis, and ESI mass spectrometry with regard to reaction conditions, intermediates, products, mechanism, and sequence specificity. Cleavage of the trioxacarcin-DNA complexes provided the natural product gutingimycin by guanine abstraction. The resulting DNA with an abasic site was further cleaved into a DNA fragment with a furanyl unit at the 3'-end and an oligonucleotide with a phosphorylated 5'-end.
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