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Dissociation-constants of metat-ion-complexes with alkaline phosphatase from pig kidney.

B P Ackermann, J Ahlers
Experientia 1976 March 16
Using metal-ion buffers it was possible to remove Zn2+, Mg2+ and Mn2+ ions of pig kidney alkaline phosphatase reversibly. The dissociation constants obtained are KEMg: 4 X 10(-7) M, KEMn: 4 X 10(-8) M and KEZn: 8 X 10(-13) M (22 degrees C, pH: 9.6, mu: 0.07).

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